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National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
Photoaffinitive conjugation of biomolecules
 Masárová, Petra ; Slanina, Tomáš (advisor) ; Vrábel, Milan (referee)
Photoclick chemistry represents a class of photo-activated click reactions generally characterised by precise spatial and temporal control, high selectivity, and quantitative yields. The following thesis will focus on several classes of light triggered click reactions and attempt to outline their chemistry in reversible nucleophilic addition, irreversible cycloaddition and Diels-Alder reactions initiated upon irradiation of photoactive precursors. The absorption of such precursors can be shifted towards visible light by derivatization of their chromophoric moieties, which allows for a non-harmful excitation in vivo and therefore offers promising prospects in biological applicability. The importance of click photochemistry resides in a remarkable selectivity that enables reactions to take place in highly complex, non-controlled biological environments. The photo-inducible precursors can be conjugated with various moieties of biological significance - a method most notably used in photoaffinity labelling. Considerable efforts have been made to explore the reactivity of photoactivatable compounds towards biological nucleophiles in various spheres of applied research which will all be discussed and summarised.
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Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy
 KOČOVÁ, Pavlína
This study is focused on changes in protein expression in a glioblastoma cell line during infection with tick-borne encephalitis virus. Newly synthesized proteins were distinguished from previously synthesized proteins using bioorthogonal chemistry (BONCAT method) to observe changes in protein synthesis. Labelled proteins were visualized using two-dimensional PAGE and western blotting followed by Click reaction on membrane. Differences in protein pattern between control and infected cells were observed.
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Identification of newly synthesised proteins in mammalian cells during tick-borne encephalitis infection using bioorthogonal chemistry (L-azidohomoalanine)
 KOČOVÁ, Pavlína
This study was focused on changes in protein expression in glioblastoma cell line after infection with tick-borne encephalitis virus. Bioorthogonal chemistry (BONCAT method) was used to distinguish between previously synthesized proteins and newly synthesized proteins to observe changes in protein synthesis. Labelled proteins were purified and visualized using SDS-PAGE and western blotting. Purified proteins were also indentified using mass spectrometry. The number of the different labelled proteins decreases as the infection progress.
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Detection and localization of fucosylated glycans in cell lines of tick \kur{(Ixodes ricinus)} using alkyne-fucose derivates.
 HUBINGEROVÁ, Hana
The aim of study was the detection and localization of fucosylated glycans in the tick Ixodes ricinus cell lines using bioorthogonal chemistry. Fucosylated glycoproteins were detected in tick cell line homogenates by SDS-PAGE and Western blotting and localized in the cell by fluorescence and electron microscopy.
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